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人腦發育標記物qPCR檢測試劑盒

英文名:Human Brain Development Markers qPCR Array Kit

貨號:GK127

價格:$250

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ScienCell's GeneQuery? Human Brain Development Markers qPCR Array Kit (GQH-BDM) surveys a panel of 24 marker genes involved in brain development, function and pathogenesis. GQH-BDM is also useful for evaluating the roles of stem and progenitor cells in brain organoid generation. Brief examples of how genes may be grouped are shown below:

  • M1 microglia phenotype markers: TNF, NOS2, IL1B
  • M2 microglia phenotype markers: IL10, ARG1, MRC1
  • Pericyte markers: ACTA2, PDGFRB, CSPG4
  • Evaluation of neural differentiation
    • Astrocyte markers:   GFAP, S100B, GLUL
    • Immature neuron markers:   NEUROD1, DCX, TUBB3
    • Mature neuron markers:   MAP2, RBFOX3, SYP
  • Neuron function evaluation: CHAT, TH (tyrosine hydroxylase), MAPT
  • Blood-brain barrier (BBB) evaluation: VCAM1, OCLN, ICAM1

Note all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg 2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

 

GeneQuery? Human Brain Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Note : all gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

TNF

NOS2

IL1B

ACTB

TNF

NOS2

IL1B

ACTB

TNF

NOS2

IL1B

ACTB

B

IL10

ARG1

MRC1

GAPDH

IL10

ARG1

MRC1

GAPDH

IL10

ARG1

MRC1

GAPDH

C

ACTA2

PDGFRB

CSPG4

LDHA

ACTA2

PDGFRB

CSPG4

LDHA

ACTA2

PDGFRB

CSPG4

LDHA

D

GFAP

S100B

GLUL

NONO

GFAP

S100B

GLUL

NONO

GFAP

S100B

GLUL

NONO

E

NEUROD1

DCX

TUBB3

PPIH

NEUROD1

DCX

TUBB3

PPIH

NEUROD1

DCX

TUBB3

PPIH

F

MAP2

RBFOX3

SYP

GDC

MAP2

RBFOX3

SYP

GDC

MAP2

RBFOX3

SYP

GDC

G

CHAT

TH

MAPT

PPC

CHAT

TH

MAPT

PPC

CHAT

TH

MAPT

PPC

H

VCAM1

OCLN

ICAM1

NTC

VCAM1

OCLN

ICAM1

NTC

VCAM1

OCLN

ICAM1

NTC

 * gene selection may be updated based on new research and development

貨號GK127
產地美國
縮寫GQH-BDM
規格One 96-well plate, one 20ul reaction per well
用途科研
儲存4°C
運輸膠冰
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