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人胸腺細胞發育標記qPCR檢測試劑盒

英文名:Human Thymocyte Development Markers qPCR Array Kit

貨號:GK117

價格:$250

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ScienCell's GeneQuery? Human Thymocyte Development Markers qPCR Array Kit (GQH-TDM) is designed to facilitate gene expression profiling of 40 marker genes involved in thymopoiesis.  The tightly regulated lineages of common lymphoid progenitors (CLPs) consist of several maturational stages based on the expression of cell surface markers, including double negative stage (CD4- and CD8-), double positive stage (CD4+ and CD8+), and single positive stage (CD4+ or CD8+). The CD4+ T cells can further differentiate into a number of effectors subsets such as Th1, Th2, Th9, Th17, and Tfh. Brief examples of how included genes may be grouped according to their functions are shown below:

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

  • CLP markers: MME, CD34, PTPRC, IL7R, FLT3
  • Double negative thymocyte markers: CD1A, CD3D, CD3E, CD3G, CD5, CD7, CD200, IL2RA
  • Double positive thymocyte markers: CD1A, CD4, CD5, CD7, CD8A, CD8B, CD38, SELL, CD200
  • CD4+ T cell markers: CD3D, CD3E, CD3G, CD4, PTPRC, IL2RA, SELL
  • CD8+ T cell markers: CD3D, CD3E, CD3G, CD8A, CD8B, GZMB
  • Regulatory T cell markers: IL2RA, ENTPD1, NT5E, ITGAE, FOXP3
  • Markers of T cell effector subsets
    • Th1: FASLG, CXCR3, CCR5, IL12RB1, IL18R1
    • Th2: CXCR3, CCR3, CCR4, CCR8, PTGDR2
    • Th9: CCR6, IL9
    • Th17: PTPRC, IL6R, KLRB1, CCR4, CCR6, IL13RA1, IL21R
    • Tfh: CXCR5, PDCD1                                              

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery? Human Thymocyte Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Note: all gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

CCR3

CD38

CD8B

IL12RB1

ITGAE

ACTB

CCR3

CD38

CD8B

IL12RB1

ITGAE

ACTB

B

CCR4

CD3D

CXCR3

IL13RA1

KLRB1

GAPDH

CCR4

CD3D

CXCR3

IL13RA1

KLRB1

GAPDH

C

CCR5

CD3E

CXCR5

IL18R1

MME

LDHA

CCR5

CD3E

CXCR5

IL18R1

MME

LDHA

D

CCR6

CD3G

ENTPD1

IL21R

NT5E

NONO

CCR6

CD3G

ENTPD1

IL21R

NT5E

NONO

E

CCR8

CD4

FASLG

IL2RA

PDCD1

PPIH

CCR8

CD4

FASLG

IL2RA

PDCD1

PPIH

F

CD1A

CD5

FLT3

IL6R

PTGDR2

GDC

CD1A

CD5

FLT3

IL6R

PTGDR2

GDC

G

CD200

CD7

FOXP3

IL7R

PTPRC

PPC

CD200

CD7

FOXP3

IL7R

PTPRC

PPC

H

CD34

CD8A

GZMB

IL9

SELL

NTC

CD34

CD8A

GZMB

IL9

SELL

NTC

 

* gene selection may be updated based on new research and development

貨號GK117
產地美國
縮寫GQH-TDM
規格One 96-well plate, one 20ul reaction per well
用途科研
儲存4°C
運輸膠冰
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