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人類適應性免疫反應qPCR檢測試劑盒

英文名:Human Adaptive Immune Response qPCR Array Kit

貨號:GK072

價格:$250

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ScienCell's GeneQuery? Human Adaptive Immune Response qPCR Array (GQH-AIR) facilitates gene expression profiling of 88 key genes involved in adaptive immunity or acquired immunity.  Vertebrates have two main immune systems: innate and adaptive.  While the innate immune system is responsible for host defense against pathogens in a general way, the adaptive immune system is responsible for defense against particular pathogens, thereby conferring immunological memory.  Brief examples of how included genes may be grouped according to their function in the adaptive immune response are shown below:

  • Antigen presentation: CANX, HLA-A, HLA-DPB1, PSMB8, TAP2
  • Th1 markers: IL12RB1, CCR5, CD94, CXCR3. IL18R
  • Th2 markers: IL13, IL1R1, IL4, CCR8, CSF2
  • Th17 markers: CCR6, IL17A, IL6, RORC, IRF4
  • Treg markers: FOXP3, CD4, IL10, IL2RA, CTLA4

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery? Human Adaptive Immune Response qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC1

CCR8

CD40LG

FOXP3

HLA-DQA1

IL12RB1

IL1R1

IRF4

NOTCH3

PTPRC

STAT6

β-actin

B

ARG1

CD19

CD80

GATA3

HLA-DQB1

IL12RB2

IL1R2

ITGAM

PDIA3

RAG1

TAP2

GAPDH

C

CANX

CD27

CD86

HAVCR2

HLA-DRA

IL13

IL2

JAK3

PSMB8

RAG2

TBX21

LDHA

D

CCL5

CD28

CSF2

HLA-A

HLA-DRB1

IL17A

IL2RA

KLRD1

PSMB9

RORA

TNF

NONO

E

CCR1

CD3D

CTLA4

HLA-B

HLA-DRB3

IL17B

IL4

LGALS1

PSME1

RORC

TNFRSF4

PPIH

F

CCR4

CD3E

CXCR3

HLA-C

IFNG

IL17C

IL5

LTA

PSME2

STAT1

TNFSF11

GDC

G

CCR5

CD3G

CXCR4

HLA-DPA1

IL10

IL18

IL6

MS4A1

PSME3

STAT3

TRAT1

PPC

H

CCR6

CD4

CXCR6

HLA-DPB1

IL12A

IL18R1

IL9

NFKB1

PTGDR2

STAT4

ZAP70

NTC

 * gene selection may be updated based on new research and development

貨號GK072
產地美國
縮寫GQH-AIR
規格One 96-well plate, one 20ul reaction per well
用途科研
儲存4°C
運輸膠冰
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