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人類乙型肝炎qPCR檢測試劑盒

英文名:Human Hepatitis B qPCR Array Kit

貨號:GK043

價格:$250

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ScienCell's GeneQuery? Human Hepatitis B qPCR Array Kit (GQH-HPB) is designed to facilitate gene expression profiling of 88 key genes involved in hepatitis B pathogenesis. Hepatitis B  is a liver infectious disease caused by the Hepatitis B virus (HBV). The HBV infection of hepatocytes triggers immune response cascades to regulate HBV replication and hepatocyte survival. Brief examples of how included genes may be grouped are shown below:     

  • HBV binding factor: HSPG2, CPD
  • Signaling pathways
    • MAPKMAPK1, MAP3K1, MAPK8, MAPK10, MAP2K1, RAF1, ELK1, YWHAB, YWHAQ, FOS
    • PI3K/AKT: AKT1, PIK3CA, PIK3CG, PIK3R1, PIK3R3, PIK3R5
    • Toll-like receptor: TLR2, TLR3, TLR4, IFNAs, IFNAR1, IFNB1
    • JAK/STAT: STAT1, STAT3, JAK1, MMP9, NFKB1, RELA, SRC
  • Genes implicated in HBV infection: LAMTOR5, RSF1, APOBEC3G, GPT, TNF, AIP, AFP, MBL2, F2

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery? Human Hepatitis B qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AFP

ATP6AP1

DDX58

HSPG2

IFNA2

IFNB1

MAP2K4

MRC1

PTMA

SRC

TIRAP

β-actin

B

AIP

CPD

EGR3

IFIH1

IFNA21

IFNG

MAP3K1

NFKB1

RAF1

STAT1

TLR2

GAPDH

C

AKT1

CREB3

ELK1

IFNA1

IFNA4

IKBKE

MAPK1

PIK3CA

RARB

STAT3

TLR3

LDHA

D

ALB

CREB3L1

F2

IFNA10

IFNA5

IRF3

MAPK10

PIK3CG

RELA

STAT6

TLR4

NONO

E

APOBEC3G

CREB3L2

FGL2

IFNA13

IFNA6

JAK1

MAPK8

PIK3R1

RFX1

TGFB1

TNF

PPIH

F

ASGR1

CTLA4

FOS

IFNA14

IFNA7

LAMTOR5

MAVS

PIK3R3

RSF1

TGFBR1

VDAC3

GDC

G

ATF4

DDB2

GGT1

IFNA16

IFNA8

MAP2K1

MBL2

PIK3R5

SLC17A5

TICAM1

YWHAB

PPC

H

ATF6B

DDX3X

GPT

IFNA17

IFNAR1

MAP2K2

MMP9

PSMA7

SON

TICAM2

YWHAQ

NTC

 

* gene selection may be updated based on new research and development

貨號GK043
產地美國
縮寫GQH-HPB
規格One 96-well plate, one 20ul reaction per well
用途科研
儲存4°C
運輸膠冰
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