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人類先天免疫反應qPCR檢測試劑盒

英文名:Human Innate Immune Response qPCR Array Kit

貨號:GK039

價格:$250

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ScienCell's GeneQuery? Human Innate Immune Response qPCR Array Kit (GQH-IIR) facilitates gene expression profiling of key genes in the human innate immune system.  The innate immune response is activated upon microbial infection or foreign substance presentation and includes activation of the complement system and adaptive immune systems.  A separate array is available for analyzing the adaptive immune response.  Brief examples of how included genes may be grouped according to function are shown below:

  • Bacteria response: TLR1, TLR5, NOD1, LY96, TRAM1
  • Virus response: TLR3, DDX58, IFIH1, MYD88, IRAK4
  • Self recognition: TLR2, TLR4, TLR7, TLR8, CLEC4E
  • Inflammasome component: CASP1, CASP5, PYCARD, NLRP1
  • Complement system component: C3, MBL2, MASP1, CFB, CD46

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery? Human Innate Immune Response qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ATF3

CASP5

CLEC6A

IFIH1

IRAK1

LYZ

NAP1L1

OTUD5

TICAM1

TLR5

TRAF6

β-actin

B

C3

CD46

CLEC7A

IFNA1

IRAK2

MAP3K7

NFAM1

PYCARD

TICAM2

TLR6

TRAM1

GAPDH

C

C5

CFB

DDX58

IFNB1

IRAK3

MAPK8

NFATC1

RIPK2

TIRAP

TLR7

TRAM2

LDHA

D

C6

CFD

DHX58

IFNG

IRAK4

MASP1

NFKB1

RNF125

TLR1

TLR8

TRIM25

NONO

E

C7

CFH

EYA4

IL18

IRF3

MASP2

NFKBIE

SYK

TLR10

TLR9

TYROBP

PPIH

F

C8A

CFP

FADD

IL1A

IRF7

MAVS

NLRP1

TAB2

TLR2

TNF

UBE2N

GDC

G

CARD9

CHUK

HCST

IL1B

JUN

MBL2

NOD1

TAB3

TLR3

TRADD

UBE2V1

PPC

H

CASP1

CLEC4E

HMGB1

IL6

LY96

MYD88

NOD2

TBK1

TLR4

TRAF3

UNC93B1

NTC

 *gene selection may be updated based on new research and development

貨號GK039
產地美國
縮寫GQH-IIR
規格One 96-well plate, one 20ul reaction per well
用途科研
儲存4°C
運輸膠冰
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