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人內吞作用qPCR檢測試劑盒

英文名:Human Endocytosis qPCR Array Kit

貨號:GK022

價格:$250

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ScienCell's GeneQuery? Human Endocytosis qPCR Array Kit (GQH-ECY) is designed to facilitate gene expression profiling of 88 key genes involved in cellular endocytosis.  Endocytosis pathways are used to mediate signal transduction from the cell surface and to internalize or transport particulate matter.  Brief examples of how included genes may be grouped according to endocytic pathway are shown below:

  • Clathrin-mediated: CLTA, CLINT1, AP2A1, SNX9, EPN1
  • Caveolae: CAV1, CAV2, CAV3, PTRF, FLOT1
  • Macropinocytosis: KRAS, SRC, PAK1, PAK2, HDAC6
  • Phagocytosis and degradation: IQGAP1, ROCK1, UBB, DCTN1, AMPH

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery? Human Endocytosis qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

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5

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7

8

9

10

11

12

A

ACTB

ARHGAP26

CLTB

DOCK1

EPN1

IQGAP1

MAPK8IP3

PIKFYVE

RAB11FIP5

RALBP1

SNX9

β-actin

B

AMPH

CAV1

CLTC

DYNAP

EPN2

KCNJ5

MYO5B

PLD2

RAB22A

RBSN

SPAG9

GAPDH

C

AP2A1

CAV2

CTTN

ECT2

EPN3

KLC1

MYO6

PRKCI

RAB35

REPS2

SRC

LDHA

D

AP2B1

CAV3

DCTN1

EEA1

EPS15

KLC2

MYOF

PTRF

RAB4A

RHOA

STX5

NONO

E

AP2S1

CDC42

DCTN2

EHD1

EXOC5

KRAS

PAK1

RAB10

RAB5A

ROCK1

SYNJ1

PPIH

F

ARF1

CEP164

DENND1A

EHD2

FLOT1

LAMP1

PAK2

RAB11A

RAB7A

SDCBP

UBB

GDC

G

ARF6

CLINT1

DNAL1

EHD3

FLOT2

MAPK1

PARD3

RAB11FIP2

RAB8A

SNX1

VPS45

PPC

H

ARHGAP10

CLTA

DNM1

EHD4

HDAC6

MAPK8IP2

PARD6A

RAB11FIP3

RACGAP1

SNX4

WWCI

NTC

 * gene selection may be updated based on new research and development

貨號GK022
產地美國
縮寫GQH-ECY
規格One 96-well plate, one 20ul reaction per well
用途科研
儲存4°C
運輸膠冰


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