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人臍靜脈內皮細胞 HUVEC

英文名:Human Umbilical Vein Endothelial Cells

貨號:8000

價格:$250

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血管內皮細胞有維持血管內環境平衡的功能,血管內皮細胞產生、分泌纖維蛋白凝集和分解系統的活化劑和抑制劑。另外它們調控血小板的粘附和聚集。內皮細胞同時釋放分子,調控細胞增殖,控制血管壁功能。人臍靜脈內皮細胞是應用最普遍的進行體外研究內皮細胞的細胞類型。臍靜脈內皮細胞顯微鏡下形態如同鵝卵石,用vWF/Factor VIII 和 CD-31容易染色,可以攝入乙酰基化低密度脂蛋白。用IL-1或TNF-α預處理后,可選擇性地表達E-selectin和VCAM。
Sciencell研究實驗室的臍靜脈內皮細胞是從人臍帶組織分離得到的,原代凍存,冰凍運輸。每管細胞密度超過5×10^5/ml。細胞特性經vWF/Factor VIII和CD31 (P-CAM) 免疫熒光鑒定,能夠攝取DiI標記乙酰化低密度脂蛋白(DiI-Ac-LDL)。本細胞經檢測不含HIV-1、HBV、HCV、支原體、細菌、酵母菌和真菌。如采用ScienCell 實驗室特制的培養基,可保證此細胞15倍增增殖。

推薦培養基:內皮細胞培養基(ECM, Cat. #1001)

貨號8000
產地美國
縮寫HUVEC
規格5 x 10^5 /1ml
用途科研
儲存液氮
運輸干冰
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3.) Chen G, Zhao L, Feng J, You G, Sun Q, Li P, Han D, Zhou H. (2013) "Validation of Reliable Reference Genes for Real-Time PCR in Human Umbilical Vein Endothelial Cells on Substrates with Different Stiffness."?PLoS One. 8: e67360

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16.) Vigl B, Zgraggen C, Rehman N, Banziger-Tobler NE, Detmar M, Halin C. (2009) "Coxsackie- and adenovirus receptor (CAR) is expressed in lymphatic vessels in human skin and affects lymphatic endothelial cell function in vitro."?Exp Cell Res. 315: 336-47.

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Question:

Greetings! I am the biosafety officer at our institution, and we've had a researcher order primary HUVEC cells from your company. Would you be able to let us know if you test the cultures/isolates for infectious agents or viruses and what agents you test for?

Answers:

Each cell strain tests negative for HIV, HBV and HCV. However, keep in mind that diagnostic tests are not necessarily 100% accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working with these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination.

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